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LEAP™ (Laser-enabled analysis and processing)
LEAP™ is an automated live cell imaging and processing system for in situ cell purification and/or laser-based transfection. LEAP™ combines high-speed imaging and multi-parameter visual characterization of cells with simultaneous laser-based cell manipulation. LEAP™ can image and process adherent and non-adherent cells using C-lect™ microplates. Capabilities of LEAP™ include:
- imaging of cells using brightfield and fluorescence characteristics; and
- laser-based manipulation to perform cell purification and/or optoinjection.
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| LEAP™. The LEAP™ system offers a truly unique and powerful means to combine live cell imaging and cell manipulation in an automated mode. Since LEAP™ exploits standard microplate formats, cell processing on LEAP™ and other automated procedures, such as cell culture and liquid handling, can be easily integrated. |
Key LEAP™ advantages include:
- laser-based delivery of a wide variety of certain molecules into cells;
- iterative cell processing to help maxamize desired cell purity and efficiency;
- laser-based manipulation of a wide variety of sample sizes;
- processing of many cell types, including fragile and primary cells; and
- safer processing of certain biohazardous cells.
C-lect™ microplates & kits
C-lect™ microplates are custom products optimized to ensure proper optical and laser compatibility with the LEAP™ system.
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| C-lect™ microplates. Cyntellect offers C-lect™ microplates that are optically matched for imaging and laser processing on LEAP™. Currently, C-lect™ plates are available in 96-well and 384-well formats, with additional formats in development. |
C-lect™ applications kits are assay kits optimized and validated for specific scientific experimentation on the LEAP™ system . Each kit comes complete with reagents, disposables, and protocols. Kits to be launched in 2006 are expected to include the following applications:
- cell-secretion profiling and purification;
- fluorescent marker-based cell purification;
- optoinjection of nucleic acids; and
- optoinjection of macromolecules.
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| C-lect™ Kits. Cyntellect offers kits optimized for performing defined applications on LEAP™. These kits include reagents, protocols and C-lect™ microplates, which are required for processing on LEAP™. |
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