Real-time Cell Characterization (or “Gating”)
The real-time imaging and gating of live cells means users can dynamically adjust gating parameters to explore how different gating criteria might improve or alter selection of “cells-of-interest”. Alternatively, the gates can be fixed for automated processing. The characteristics of each cell can be queried while the living cells are maintained in situ, a novel capability in HT cell analysis. Then, based upon the experimental design, the laser can be fired at cells with certain of these properties. For example, if a researcher interested in evaluating chemosensitivity had live cells derived from a patient biopsy sample, stained for a specific disease marker, the laser could be fired at all cells not expressing that marker, thereby leaving a purified population of diseased cells for accurate assessment of chemosensitive response.

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Real-time & Dynamic Image-based Gating. LEAP™ software allows selection of a wide variety of image-based gating criteria to identify cells-of-interest. Histogram plots and meta-images display the actual cells being imaged and cells-of-interest are highlighted. Thumbnail images of the “best fit” cells are presented for visual analysis. The real-time imaging and gating of live cells means users can dynamically adjust gating parameters to explore how alternate gating criteria might improve or alter selection of “cells-of-interest”. Alternatively, the gates can be fixed for automated processing.

Using this approach, cells can be profiled based on a wide variety of visual parameters enabling identification of the precise cell or cells that meet the target functional or phenotypic specifications. 

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