Confluency Measurement

The Celigo enables automated, label-free, non-destructive, and non-invasive determination of confluence and growth characteristics of cell lines, including newly established rare, and difficult-to-grow cell lines. These samples can be monitored without any manipulation of the cells, permitting cultures to be quantified in situ and allowing multiple accurate reads of the same culture over time. Celigo provides a unique combination of rapid brightfield imaging, image segmentation, and analysis tools for researchers to monitor cell cultures and generate growth curves over time, identifying doubling rates, and doubling times. The confluence application on Celigo provides the following benefits:

• Scanning and analysis of multi-well plates (1536-well to 6-well) and T-flasks (T-25 and T-75)
• Rapid scanning and analysis of multi-well plates (384-well plate can be analyzed in <8 min)
• Analysis of cells growing in T-25 and T-25 flasks (T-75 flasks can be analyzed in ~15 min)
• Easy-to-use interface to acquire and analyze images.
• Reports confluence, growth curves, doubling time, and doubling rate for each well

Label-free brightfield growth analysis on Celigo. Zoomed brightfield images (top row) and analysis overlay (bottom) of adherent cells (HeLa) over multiple days in 96-well microplate.

Cell growth characteristics measured on Celigo. Confluence application reports label-free growth curves for individual wells (top left) and multi-well thumbnails (top right), as well as, doubling time (bottom left) and doubling rate (bottom right).

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