Multi-Parameter Gating

The Celigo software provides a rapid solution for multi-channel analysis of brightfield and fluorescent images and a means for cell identification based on intensity and morphology parameters. Moreover, a powerful, yet easy-to-use, gating interface allows the analysis of cell populations similarly to flow cytometry. Specifically, once fluorescent or brightfield images are segmented into objects, a set of gates based on morphology or intensity is used to define populations and classes of cells for which statistical data is reported. The multi-parameter gating functionality of the Celigo software provides the following benefits:

• Definition of an unlimited number of plots and cell populations
• Interactive, real-time classification of cell populations using histogram and scatter plots
• Multiple range and splitter gates on histogram plots
• Quadrant and multiple polygon gates on scatter plots
• Combination of gates using a full set of Boolean operators
• Identification of cell classes for which statistical data is reported for all wells

Celigo gating interface. Following analysis of brightfield and fluorescent images, the celigo software allows histogram (upper left) and scatter plot (lower plots) representation of the segmentation data. Unlimited plots, gates and cell populations can be identified and cells can be classified using a color overlay (upper right).