Cell Counting

Celigo’s cell counting application, which features whole-well and label-free brightfield imaging, dramatically improves the counting accuracy of cell cultures and eliminates the need for detachment enzymes and hematocytometers to determine cell culture density. The flexibility and ease-of-use of the Celigo cell counting application also allows cell growth to be monitored over time and facilitates the daily management of cultures. The standardization of this process leads to increased consistency of quantitative cell-based assays. The benefits of the cell counting application on Celigo include:

• Rapid brightfield imaging and label-free in situ analysis of cells
• Whole-well image capture with a 1 u/pixel resolution
• Imaging of multi-well plates (1536-well to 6-well) and T-flasks (T-25 and T-75)
• Easy-to-use interface to image and identify a wide variety of cell types in brightfield
• Straight-forward image segmentation and cell identification software
• Graphic and numeric reports of growth curves, cell counts, doubling time, and doubling rates

Cell counting of adherent cells on Celigo. MDA-MB-436, MCF7, and MDA-MB-231 cell lines imaged (top) and segmented (bottom) using the Direct Cell Counting application.

Cell counting of suspension cell on Celigo. CHOs, Jurkat and THB-5 cell lines imaged (top) and segmented (bottom) using the Direct Cell Counting application.

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