Proliferation Assay
Assessing the proliferative activity of cells grown in culture is a fundamental tool for monitoring the health and growth rate of cells. Many researchers use metabolic-based quantification methods, such as MTT or MTS assays, to evaluate and identify compounds affecting cell proliferation. Unfortunately, these end-point assays require one to accept the measurement of metabolic activity as an indirect indicator for cell number and are destructive requiring the use of a new sample for each time point. Celigo enables automated, label-free, non-destructive, and non-invasive proliferation assays with its Cell Counting application. Cell counting on Celigo confers several benefits:
- Image and count cells directly in situ
- Label-free cell counting requires no reagents
- Accurate, whole-well brightfield imaging and segmentation counts every cell in every well
- Multiple reads of the same sample reduce cost and effort
- Accurate normalization of wells using the actual number of cells in each well
- Allows for morphological inspection and analysis
Celigo vs. MTT assay comparison. Celigo analyzes cells at multiple time points and directly counts cells in situ using live brightfield images to accurately measure cell proliferation. MTT assays are analyzed at 1 time point and indirectly estimate cell proliferation.
Brightfield images of Colo-205 cells on Celigo. Cells were treated with Compound X (left) and 5-FU (right) and images were acquired at multiple time points. After 5-FU treatment, a morphological change is observed at time point 3.
Concentration response curve generated using Celigo. Image analysis of Colo-205 cells over time in response to compound treatment (left, middle) acquired on Celigo. MTT analysis of Colo-205 cells at a single time point (right).
Featured Solutions
Our Products
Support
Commitment to Our Customers
Experienced scientific and technical customer support teams are ready to advise with timely and professional assistance.