Cell Line Characterization

Determination of the growth characteristics of primary cells and cell lines can be accurately analyzed in only a few minutes using the Celigo. The Celigo provides a unique combination of brightfield imaging, image segmentation, and analysis tools for researchers to monitor their cell cultures. Specifically, whole-well imaging and label-free brightfield cell counting allows the Celigo user to generate growth curves over time and monitor cell counts, confluency, doubling rate, and doubling time at the individual well level. Direct cell counting on Celigo provides several benefits:

• Rapid scanning and analysis of multi-well plates (384-well plate can be analyzed in <5 min)
• Easy-to-use interface to image and identify a wide variety of cells in brightfield
• Determine growth characteristics of cells in situ, directly where they are grown
• Reports growth curves, cell counts, confluency, doubling time, and doubling rate for each well
• Analyze cells growing in T-flasks (T-25 and T-75), T-75 flasks can be analyzed in ~15 min

Label-free cell growth analysis on Celigo. Zoomed brightfield images of segmented HeLa cells over time using the Cell Counting application which can be used to analyze cell counts (top) and confluency (bottom).

Cell growth characteristics measured on Celigo. The direct cell counting application reports label-free growth curves for individual wells (top left) and multi-well plates (top right). It also reports doubling time (bottom left) and doubling rate (bottom right) for each well of a plate.

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