Cell Health

Quick assessment of cell health can be performed using the Celigo, which has easy-to-use applications that allow the simple and rapid analysis of cell viability and apoptosis in many diverse cell types. The Cell Viability application uses calcein AM, propidium iodide, and Hoechst to identify and count live, dead, and total cell numbers, respectively. Similarly, the Phosphatidylserine (PS) Externalization application measures apoptosis by quantifying annexin V, propidium iodide, and Hoechst to report apoptotic, dead, and total cell counts, respectively. Analysis of Cell Health on Celigo has several advantages:

• Easy-to-use interface to identify cell health
  
• Rapid scanning and analysis (scan and analyze a 96-well plate using 3 fluorescent channels in <12 min)
  
• Works in multi-well plate formats (1536-well to 6-well)
  
• Advanced flow cytometry-like gating

Cell viability application on Celigo. Representative image and concentration response curve of HeLa cells treated with hydrogen peroxide.

Phosphatidylserine (PS) externalization application on Celigo. Representative image and concentration response curve of apoptotic Jurkat cells treated with camptothecin.

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