Webinars

Standardizing Stem Cell Cultures to Maximize Differentiation Potential

Speaker:

Kristi Hohenstein, PhD.
Cyntellect

• View Webinar Now >

Abstract

Derivation and propagation of embryonic and induced pluripotent stem cell (ESC/iPSC) lines are common tasks in stem cell biology, yet typical procedures vary extensively among laboratories. Manual methods are generally preferred because they result in consistent stem cell cultures, whereas enzymatic techniques are more widely used due to their scalability, but at the expense of reproducibility. To address these challenges, Cyntellect has developed innovative stem cell applications using the LEAP™ cell processing Workstation. More specifically, the Stem Cell Passage application is a novel approach for efficient, standardized passage of embryonic stem cell cultures. LEAP uses a laser to section undifferentiated stem cell colonies into cell clusters of defined size, resulting in more uniform colonies than current methodologies. A second novel application, EB Generation, provides the direct generation of embryoid bodies of specific sizes. This unique application is used to physically section stem cell cultures into defined sizes for the purpose of generating homogenous, uniformly-sized EB populations. Results demonstrate specific sized EBs differentiate more efficiently into neurons and cardiomyocytes than typical heterogeneous EB cultures. These novel applications provide automated, reproducible methods for propagation of large-scale ESC/iPSC cultures and can significantly improve the efficiency of ESC/iPSC differentiation for generation of specialized cell types for cell-based screening and therapeutics.

• View Webinar Now >