Synopsis

The LEAP™ Workstation is a microplate-based cytometry system for in situ cell purification, processing and analysis of virtually any cell type. Combining high-speed whole well imaging and ultra fast laser manipulation for selective and efficient processing of cells in their natural state, the LEAP workstation is the ideal precision tool for purifying and processing both adherent and non-adherent cell types in a variety of applications.

Benefits

• Purify and analyze cell cultures in a closed, sterile microplate environment
• Purify adherent or non-adherent cells right where they grow
• Clone or enrich cell populations based on imaging phenotype
• Analyze with the versatility of fluorescence and/or label-free brightfield imaging

LEAP Systems

LEAP – Life Science Research - Automated High-Throughput in situ Cell Purification

• Clone or enrich cell population based on phenotype
• Purification of adherent cells In Situ while still adherent to culture substrate
• Purify small cell samples with high yield and purity

LEAP – Stem Cell Manager - Automated Stem Cell Culture Management

• Provide rapid, automatable, enzyme-free, physical passage of ES and iPS cells
• Generate reproducible stem cultures with more uniform colony size distribution
• Generate EBs of specific size for improved differentiation of specialized cell types
• Purify colonies with laser precision

LEAP – CellXpress™ - Rapid High Secretion Cell Line Development (Therapeutic)

• Identify and quantify the highest secreting single cells with live cell secretion assay
• Instant laser based purification of highly secreting clones
• Quantify and rank secretors based on growth characteristics
• Monitor and identify stable secreting clone populations

Applications available

1. Cell Purification
2. Stem Cell Manager includes:
   1. Stem Cell Passage
   2. EB Generation
3. CellXpress includes:
   1. Secretion Cloning
   2. Growth Tracking
   3. Secretion Stability Tracking
4. Cell Secretion
5. Cell Viability
6. Cell Counting
7. Manual Laser Targeting

Brightfield source

LED

Fluorescence source

Metal halide (200 W, 1500 hr)

Fluorescence filters

8 position excitation, dichroic and emission filter wheels

Detection modes

Fluorescence & Enhanced Uniform Brightfield

Detector

Cooled emCCD (1024 x 1024 pixels, 8 µm pixel)

Plate formats

C-lect™ brand 384, 96 (image & process), 12, 6 wells (process only)

Plate scan times

20 min (Whole well imaging; 2 channels, 384 well plate)

Magnification

3x, 5x, 10x, 20x (0.25 NA, F-Theta lens)

Imaging area

6.5 mm - 1 complete well of 96-well plate

Image resolution

3x – 2 µm/pixel; 5x – 1.2 µm/pixel; 10x – 0.6 µm/pixel; 20x- 0.3 µm/pixel

Focus mechanism

Image-based autofocus

Cell processing lasers

355 nm and 532 nm solid state lasers

Laser processing speed

Up to 1000 targets/sec

Laser spot size

10 µm – 50 µm diameter

Cell purity and yield

Typically >99% purity and >90% yield

Computer hardware

Dell, Quad Core Processor, 1 TByte Storage, Windows XP, Dual Flat Panel monitors

Image output

Image export file format: TIFF

Dimensions

Size 40”x 29”x 24”; Weight: 400 lb (102 x 74 x 61 cm; 180 kg)

Requirements

110-220 VAC, 50-60 Hz, 20A

• Specification Sheet - LEAP™ Cellular Purification and Processing Workstation
• Application Note - Optoinjection of siRNA and functional knockdown of erythropoietin receptor in primary human CD34+ cells
• Application Note - Automated in situ purification of primary rat brain microvascular endothelial cells
• Application Note - Automated high-throughput wound healing assay
• Application Note - In situ purification of human embryonic stem cell colonies
• Application Note - High-throughput stable cell line generation
• Application Note - CellXpress™ powered by LEAP accelerated development of highly-secreting cell lines
• Application Note - Automated stem cell passage powered by LEAP physical passage of ES and iPS cell colonies
• Application Note - Automated embryoid body generation powered by LEAP generation of EBs of specific size