LEAP Analytics

The LEAP software provides a suite of integrated tools for micro-plate based cellular analysis, purification, and manipulation – from application selection and image acquisition to real time gating and processing. The software utilizes a workflow where predefined applications can be selected by the user.

A typical purification protocol involves

Step 1 Selection of application

Select the purification application from a scroll down menu and edit plate map or load a pre-defined platemap setting.

Selecting an application activates an intuitive step through software user interface.

Step 2 Real time gating

Acquire a multi wave length image of the whole well, identify cells of interest using fluorescence or morphology based parameters. Select the cells you want to keep based on user defined single or multiple gating parameters. Apply the settings to the desired wells before processing.

Real-time & Dynamic Image-based Gating. LEAP software allows selection of a wide variety of image-based gating criteria to identify cells-of-interest. Histogram plots and meta-images display the actual cells being imaged and cells-of-interest are highlighted. Thumbnail images of the “best fit” cells are presented for visual analysis. The real-time imaging and gating of live cells means users can dynamically adjust gating parameters to explore how alternate gating criteria might improve or alter selection of “cells-of-interest”. Alternatively, the gates can be fixed for automated processing.

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Step 3 Results viewer

A post processing plate map allows the user to view the results at single well level for further analysis or processing.