Celigo® Specifications

Features and Benefits

• Accepts T-flasks (T-25 and T-75) and most multi-well plates (1536-well to 6-well)
• Extremely accurate brightfield cell imaging and identification across the entire well
• Three channel fluorescence (in addition to brightfield)
• Extremely rapid scanning (~5-15 min across a range of microplates)
• Intuitive and easy-to-use, yet powerful, software segmentation and gating interface
• Optional API software for robotic loading integration

Applications

Brightfield Label-Free Assays

• Cell counting & growth tracking
  • useful for cell line characterization, clonal validation, and morphology based screening
• Colony counting: Sphere analysis
  • useful for EB characterization and tumor sphere analysis
• Confluency
  • useful for cell line characterization and infectivity assays

Fluorescence Assays

• Cell counting & growth tracking
• useful for cell line characterization, clonal validation, and morphology based screening
• Cell secretion assay
• useful for cell secretion analysis
• Cell viability
• useful for assay development, cell health assays, and high-throughput screening
• DNA synthesis
• useful for cell cycle analysis, cell health assays, high-throughput screening, and proliferation assays
• Expression analysis
• useful for cell signaling, high-throughput screening, marker analysis, and transfection optimization
• PS externalization
• useful for assay development, cell health assays, and high-throughput screening

Cell Analysis Parameters

• Total Cell Number
• Gated Cell Number
• Percentage of Gated Cells
• Cell Density
• Cell Area
• Cell Mean Intensity
• Cell Integrated Intensity
• Cell Aspect Ratio
• Cell Form Factor
• Cell Smoothness
• Colony Diameter
• Colony Perimeter

Plate Formats

• 1536-, 384-, 96-, 24-, 12-, and 6-well plates
• T-25 and T-75 flasks

Plate Scan Time

• As little as 5 - 15 min across a range of microplate formats

Resolution

• 1um/pixel; 0.25 NA full-resolution (optional 2 x 2 binning mode)

Detector

• CCD (2052 x 2052 pixels)

Detection Modes

• LED-based enhanced brightfield imaging with uniform illumination across entire well
• LED-based fluorescent imaging with 3 excitation and emission filter sets

Fluorescence Detection Range

• In addition to brightfield, 3 excitation and emission filters to provide up to 4 channels of image data
• Fluorescent wavelengths include:

Channel	Excitation	Emission	Common Dyes
1-Blue	377	447	Hoechst, DAPI
2-Green	483	536	FITC, Calcein, Alexa 488
3-Red	531	629	PI, Texas Red, Alexa 568

Focus mechanism

Image-based autofocus and rapid image-independent autofocus

Computer Hardware

• Dell Precision T3500 or similar
• USB 3.0 external Hard Drive for additional data storage

Software Output

• Image export file format: TIFF, JPEG, BMP
• Image format for database storage: JPEG 2000

Dimensions

• Enclosure Dimensions: 19” X 25” X 20” (48 cm x 64 cm x 51 cm)
• Weight: 117 lb (53 kg)

Power Requirements

110-220 VAC 50-60 Hz (dedicated circuit with 3 outlets)