Synopsis

The Celigo™ adherent cell cytometer enables in situ brightfield and fluorescent analysis of adherent cells with minimal sample manipulation. The system measures multiple cell-based parameters providing significant flexibility.

Benefits

• Analyze cell cultures in situ, with minimal disturbance
• Eliminate enzymatic disruption of sample during preparation procedures
• Image cells in brightfield and fluorescence on the same platform
• Analyze every cell in every well in brightfield mode with no ‘edge effect’

Applications

Brightfield Label-Free Assays for Live Cells

• Cell counting for adherent cells and for non-adherent cells
• Cell growth tracking
• Colony counting

Fluorescence Assays

• Cell counting
• Cell viability (toxicity)
• DNA synthesis (cell proliferation)
• PS externalization (apoptosis)

Cell Secretion Assay

• Measurement of antibody secretion from individual cells

Specifications

Cellular Analysis Parameters

• Total Cell Number (all cells)
• Gated Cell Number (cells within user specified gates)
• Average Cell Density (cells/well)
• Average Cell Area Density (cells/cm²)
• Average Cell Diameter (µm)
• Average Cell Area (µm²)
• Average Cell Perimeter (µm)

Detection Modes

• LED-based enhanced brightfield imaging with uniform illumination across well
• LED-based fluorescent imaging with 3 excitation filters and 3 emission filters

Plate Formats

• 384, 96, 24, 12, 6 well plates (coming soon 1536 well plates)
• T-25 and T-75 flasks

Plate Scan Time

As little as 5 - 15 min across a range of microplate formats

Magnification

3.5x magnification (1µm/pixel; 0.25 NA)

Fluorescence Detection Range

3 excitation filters and 3 emission filters, to allow for 1, 2, or 3 channels of image data. Multiple channels of fluorescence data can be a combination of brightfield and fluorescence, which will allow 3 fluorescence and 1 brightfield channels.

Computer Hardware

Dell Precision T3500 or similar

Software Output

• Image export file format: TIFF
• Image format for database storage: JPEG 2000

Dimensions

• Enclosure Dimensions: 19” X 25” X 20” (48cm x 64cm x 51cm)
• Weight: 117lb (53kg)

Power Requirements

110-220 VAC 50-60 Hz

• Brochure – Celigo™ Adherent Cell Cytometer
• Specification Sheet - Celigo™ Adherent Cell Cytometer
• Application Note - Automated, label-free growth tracking and culture management within flasks and multi-well plates
• Application Note - Rapid, label-free measurement of cell proliferation for compound screening
• Application Note - Rapid, cell-based in situ cell health assessments
• Application Note - Measurement of antibody secretion from Individual cells for biomanufacturing
• Application Note - Rapid, label-free counting and characterization of live embryoid bodies